Brucellosis is a bacterial infection that can be transmitted to humans from infected animals or contaminated animal products. The infection can cause a range of symptoms, including fever, headache, muscle pain, joint pain, and fatigue. Brucellosis can be diagnosed using a variety of tests, including antigen-based tests. Antigen-based tests detect the presence of Brucella-specific antigens in the blood or other body fluids of a person suspected of having brucellosis. The test involves collecting a blood sample from the patient and sending it to a laboratory for analysis. There are several types of antigen-based tests for brucellosis, including the Rose Bengal test, the Brucella microagglutination test (MAT), and the enzyme-linked immunosorbent assay (ELISA). The Rose Bengal test is a rapid screening test that detects antibodies to Brucella in the blood, while the MAT and ELISA are more sensitive tests that can detect smaller amounts of antigens and antibodies. Antigen-based tests for brucellosis are useful for diagnosing acute cases of the infection, but they may not be as reliable for detecting chronic cases or for monitoring the effectiveness of treatment. False-positive and false-negative results can occur, so it is important to confirm positive test results with additional tests. If you have symptoms of brucellosis or have been exposed to infected animals or animal products, you should talk to your healthcare provider about getting tested for the infection. Early diagnosis and treatment of brucellosis can help to prevent complications and improve outcomes.